1MRD

PREPARATION, CHARACTERIZATION AND CRYSTALLIZATION OF AN ANTIBODY FAB FRAGMENT THAT RECOGNIZES RNA. CRYSTAL STRUCTURES OF NATIVE FAB AND THREE FAB-MONONUCLEOTIDE COMPLEXES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	66.200, 75.300, 105.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.300
R-factor	0.195
Rwork	0.195
RMSD bond length	0.010
RMSD bond angle	1.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.070 *
Total number of observations	82347 *
Number of reflections	21045 *
Completeness [%]	90.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000
2	1	1	Tris-HCl