1MR8
MIGRATION INHIBITORY FACTOR-RELATED PROTEIN 8 FROM HUMAN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-01 |
| Detector | FUJI |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 52.500, 52.500, 128.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.334 |
| Total number of observations | 97834 * | |
| Number of reflections | 16285 * | 2129 * |
| <I/σ(I)> | 10.1 | 2.1 |
| Completeness [%] | 96.3 | 88.5 * |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 291 * | 15%(W/V) PEG6000, 0.1M MES, 0.1M MES PH 6.2, 20MM CACL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 15 (%(w/v)) | |
| 3 | 1 | reservoir | MPD | 7 (%(v/v)) | |
| 4 | 1 | reservoir | MES | 0.1 (M) | |
| 5 | 1 | reservoir | 20 (mM) |
