1MQS
Crystal structure of Sly1p in complex with an N-terminal peptide of Sed5p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9875,0.9793,0.9789,0.9184 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 161.056, 161.056, 88.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| Rwork | 0.257 |
| R-free | 0.29040 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.044 | 0.552 |
| Total number of observations | 200527 * | |
| Number of reflections | 26182 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 3.5M sodium formate, 20mM Tris HCl pH 7.2, 50mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 3.5M sodium formate, 20mM Tris HCl pH 7.2, 50mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | sodium formate | 3.4-3.6 (M) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | pH7.2 |
