1MP8

Crystal structure of Focal Adhesion Kinase (FAK)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	45.189, 46.636, 62.225
Unit cell angles	90.00, 80.57, 90.00

Refinement procedure

Resolution	46.000 - 1.700 *
R-factor	0.18182
Rwork	0.179
R-free	0.23000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.660 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	1.700
High resolution limit [Å]	1.700 *	1.600
Rmerge	0.071 *	0.255 *
Number of reflections	24650 *
Completeness [%]	91.1 *	65 *
Redundancy	3.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6 *	4 *	PEG 2000, citrate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.1 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.6
3	1	drop		250 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop	dithiothreitol	1 (mM)
6	1	reservoir	citrate/acetate	0.1 (M)	pH5.
7	1	reservoir	PEG2000 MME	24 (%)