1MP4

W224H VARIANT OF S. ENTERICA RmlA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Collection date	2001-06-08
Wavelength(s)	1.1395
Spacegroup name	P 65 2 2
Unit cell lengths	88.958, 88.958, 329.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 * - 2.200
R-factor	0.2838811
Rwork	0.257
R-free	0.28900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iin
RMSD bond length	0.007 *
RMSD bond angle	1.260 *

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200 *
Rmerge	0.061 *
Number of reflections	43514
Completeness [%]	94.3 *
Redundancy	17.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *		pH 7.0, temperature 100K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	drop		10 (mM)
3	1	drop	HEPES	5 (mM)	pH7.4
4	1	reservoir	sodium phosphate	0.8 (M)
5	1	reservoir	potassium phosphate	0.8 (M)
6	1	reservoir	HEPES	0.1 (M)	pH7.5