1MP3
L89T VARIANT OF S. ENTERICA RmlA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Collection date | 2001-03-29 |
Wavelength(s) | 1.0720 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 120.015, 120.015, 93.898 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 * - 2.210* |
Rwork | 0.240 |
R-free | 0.28100 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB EMTRY 1IIN |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.300 * |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.049 * |
Number of reflections | 33576 |
Completeness [%] | 95.0 * |
Redundancy | 4. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | pH 7.0, temperature 100K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15-20 (mg/ml) | |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | HEPES | 5 (mM) | pH7.4 |
4 | 1 | reservoir | ammonium phosphate | 2.0 (M) | |
5 | 1 | reservoir | 10 (mM) | ||
6 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |