1MOZ
ADP-ribosylation factor-like 1 (ARL1) from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 |
| Unit cell lengths | 104.250, 104.250, 45.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 - 3.170 |
| R-factor | 0.24 |
| Rwork | 0.206 |
| R-free | 0.24200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rrf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 3.220 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmerge | 0.071 | 0.217 |
| Total number of observations | 30535 * | |
| Number of reflections | 9179 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 97.5 | 94.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | polyethylene glycol 8000, TRIS, GDP, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 20 (%) | |
| 2 | 1 | reservoir | glycerol | 15 (%) | |
| 3 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
| 4 | 1 | drop | protein | 1 (mM) |
