1MOV

Crystal structure of Coral protein mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-06-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	I 41 2 2
Unit cell lengths	131.391, 131.391, 150.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.400
R-factor	0.225
Rwork	0.225
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mou
RMSD bond length	0.006 *
RMSD bond angle	1.335 *
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.071	0.335
Total number of observations	176177 *
Number of reflections	25988
<I/σ(I)>		1.9
Completeness [%]	99.7	99.7
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2 *	293	peg, tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir	PEG3350	10-15 (%)
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.2
4	1	reservoir	potassium iodide	0.2 (M)