1MN8
Structure of Moloney Murine Leukaemia Virus Matrix Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9777 |
Spacegroup name | P 1 |
Unit cell lengths | 33.800, 49.500, 50.800 |
Unit cell angles | 71.90, 81.90, 80.00 |
Refinement procedure
Resolution | 20.000 - 1.000 |
R-free | 0.16900 |
Structure solution method | MIR |
RMSD bond length | 0.016 |
RMSD bond angle | 2.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.040 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.062 | |
Number of reflections | 141760 | |
<I/σ(I)> | 18.1 | 0.9 |
Completeness [%] | 86.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-Na pH 7.5, 1.4M sodium citrate, 100mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-Na pH 7.5, 1.4M sodium citrate, 100mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3 (mg/ml) | |
2 | 1 | reservoir | HEPES-Na | 0.1 (M) | pH7.5 |
3 | 1 | reservoir | sodium citrate | 1.4 (M) | |
4 | 1 | reservoir | 100 (mM) |