1MMI

E. COLI DNA POLYMERASE BETA SUBUNIT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-23
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	80.012, 66.595, 80.779
Unit cell angles	90.00, 114.02, 90.00

Refinement procedure

Resolution	50.000 - 1.850 *
R-factor	0.19119
Rwork	0.188
R-free	0.24460 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pol
RMSD bond length	0.008
RMSD bond angle	1.370
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.056	0.467
Number of reflections	62043	6056 *
<I/σ(I)>		1.75
Completeness [%]	93.4 *	91.7
Redundancy	1.92	1.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	294 *	100 mM Na/H MES, 50 to 60 mM CaCl2, 30% v/v PEG 400, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	45-60 (mg/ml)
2	1	reservoir	sodium MES	100 (mM)	pH6.0
3	1	reservoir		50-60 (mM)
4	1	reservoir	PEG400	30 (%(v/v))