1MME
THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.980, 64.980, 138.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 3.100 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.29300 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 16.500 * |
| Data reduction software | IPMOSFLM |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 3.100 |
| Rmerge | 0.085 |
| Number of reflections | 6529 |
| Completeness [%] | 99.9 |
| Redundancy | 3.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 20 * | pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | GLYCEROL | ||
| 4 | 1 | 1 | NH4 CACODYLATE | ||
| 5 | 1 | 1 | NH4 ACETATE | ||
| 6 | 1 | 1 | MG ACETATE | ||
| 7 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ribozyme complex | 0.5 (mM) | |
| 2 | 1 | drop | ammonium cacodylate | 10 (mM) | |
| 3 | 1 | reservoir | ammonium cacodylate | 50 (mM) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | spermine | 1 (mM) | |
| 7 | 1 | reservoir | PEG6000 | 23 (%) | |
| 8 | 1 | reservoir | glycerol | 5 (%) |
