1MM8
Crystal structure of Tn5 Transposase complexed with ME DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-22 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.96486 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 112.500, 112.500, 233.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 500.000 - 2.900 * |
| Rwork | 0.217 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.800 |
| High resolution limit [Å] | 2.900 * | 1.900 |
| Rmerge | 0.145 | |
| Total number of observations | 299225 * | |
| Number of reflections | 22984 | |
| Completeness [%] | 90.0 | 50 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 * | 293 | 15% Peg 1500, 300 mM Na tartrate, 50 mM Hepes, 5 mM MnCl2, pH 8., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Peg 1500 | ||
| 2 | 1 | 1 | Na tartrate | ||
| 3 | 1 | 1 | Hepes | ||
| 4 | 1 | 1 | MnCl2 | ||
| 5 | 1 | 2 | Na tartrate | ||
| 6 | 1 | 2 | MnCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 300 (mM) | ||
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.7 |
| 3 | 1 | drop | EDTA | 2 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG1500 | 15 (%(w/v)) | |
| 6 | 1 | reservoir | sodium tartrate | 300 (mM) | |
| 7 | 1 | reservoir | HEPES | 50 (mM) | pH8.0 |
