1MLY
Crystal Structure of 7,8-Diaminopelargonic Acid Synthase in complex with the cis isomer of amiclenomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.968 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.655, 56.073, 116.174 |
| Unit cell angles | 90.00, 109.86, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.810 |
| R-factor | 0.19572 |
| Rwork | 0.194 |
| R-free | 0.22000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.376 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.062 * | 0.259 * |
| Total number of observations | 276229 * | |
| Number of reflections | 69507 * | |
| <I/σ(I)> | 17.4 | 3.4 |
| Completeness [%] | 99.0 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 294 | Kack, H., (1998) Acta Crystallogr., D54, 1397. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 21 (%) | |
| 3 | 1 | reservoir | 2-propanol | 12 (%) |
