1ML9
Structure of the Neurospora SET domain protein DIM-5, a histone lysine methyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0332, 1.2834, 1.2830 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.730, 81.560, 101.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.830 - 1.980 |
| Rwork | 0.205 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.830 | 2.020 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.055 | 0.276 |
| Total number of observations | 112569 * | |
| Number of reflections | 20963 | |
| <I/σ(I)> | 15.4 | 6.1 |
| Completeness [%] | 97.0 | 95.8 |
| Redundancy | 5.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.8 * | 16 * | ammonium sulfate, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | glycine | 20 (mM) | pH9.8 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | glycerol | 5 (%) | |
| 6 | 1 | drop | AdoHcy | 0.600 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 1.1-1.2 (M) | |
| 8 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.4-5.6 |
