1MKY

Structural Analysis of the Domain Interactions in Der, a Switch Protein Containing Two GTPase Domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	62.137, 77.994, 96.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.900
R-factor	0.204
Rwork	0.204
R-free	0.22700 *
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	22.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.990
High resolution limit [Å]	1.900	1.900
Rmerge	0.074 *	0.344 *
Total number of observations	465562 *
Number of reflections	44093 *
<I/σ(I)>	26.5	6.7
Completeness [%]	99.6	99.2 *
Redundancy	10.6	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	298	sodium phosphate, potassium phosphate, 3-(cyclohexylamino)-1-propane sulphonic acid (CAPS), pH 10.0, VAPOR DIFFUSION, HANGING DROP at 298K, temperature 298K, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		1.3 (M)
2	1	reservoir		0.7 (M)
3	1	reservoir	CAPS	50 (mM)	pH10.0
4	1	reservoir	beta-mercaptoethanol	15 (mM)
5	1	drop	protein	26 (mg/ml)
6	1	drop		0.8 (M)
7	1	drop		0.5 (M)
8	1	drop	CAPS	50 (mM)	pH10.0
9	1	drop	beta-mercaptoethanol	15 (mM)