1MJS
MH2 domain of transcriptional factor SMAD3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Detector technology | CCD |
| Collection date | 2000-06-17 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 54.325, 54.325, 59.428 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.120 - 1.900 * |
| Rwork | 0.196 |
| R-free | 0.20400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SMAD4 active fragment |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.120 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.210 |
| Total number of observations | 64905 * | |
| Number of reflections | 14785 * | |
| <I/σ(I)> | 20.2 | 3.53 |
| Completeness [%] | 96.4 | 77.7 * |
| Redundancy | 4.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium dihydrogen phosphate | 700 (mM) | |
| 2 | 1 | reservoir | ethylene glycol | 20 (%) | pH4.8 |
| 3 | 1 | drop | Tris | 20 (mM) | pH7.4 |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | EDTA | 0.1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) |
