1MIJ
Crystal Structure of the Homeo-prospero Domain of D. melanogaster Prospero
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2001-06-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9790, 0.9793, 0.9611 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.300, 49.900, 51.500 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.050 |
| Rwork | 0.216 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 500.000 |
| High resolution limit [Å] | 2.050 |
| Rmerge | 0.060 * |
| Number of reflections | 11045 |
| Completeness [%] | 98.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 323 | PEG 4000, Tris, n-tetradecyl beta-D-maltoside, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 323K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.8 (mg/ml) | |
| 2 | 1 | drop | HEPES | 15 (mM) | pH7.9 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | reservoir | PEG4000 | 21 (%) | |
| 6 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 7 | 1 | reservoir | n-tetradecyl-beta-D-maltoside | 0.01 (mM) |
