1MIH

A ROLE FOR CHEY GLU 89 IN CHEZ-MEDIATED DEPHOSPHORYLATION OF THE E. COLI CHEMOTAXIS RESPONSE REGULATOR CHEY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-06-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	53.504, 53.615, 160.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 * - 2.700
R-factor	0.243
Rwork	0.226
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1FQW
RMSD bond length	0.006 *
RMSD bond angle	1.230 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.044 *	0.213 *
Total number of observations	87060 *
Number of reflections	13060
<I/σ(I)>	27.2	6.5
Completeness [%]	98.3 *	98
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293	1.8 M Ammonium sulfate, 1 mM MnCl2, 10 mM NaF, 1 mM BeCl2, 5% glycerol, 10 mg/ml CheY N59R, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		1 (mM)
3	1	drop		10 (mM)
4	1	drop		1 (mM)
5	1	reservoir	Tris	100 (mM)	pH8.4
6	1	reservoir	ammonium sulfate	1.8 (M)
7	1	reservoir	glycerol	5 (%(v/v))