1MI0

Crystal Structure of the redesigned protein G variant NuG2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 1 2 1
Unit cell lengths	47.330, 73.790, 39.180
Unit cell angles	90.00, 96.00, 90.00

Refinement procedure

Resolution	15.000 * - 1.850
R-factor	0.291
Rwork	0.260
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	3.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.840	1.930
High resolution limit [Å]	1.850	1.850
Rmerge	0.067 *
Number of reflections	10905 *
Completeness [%]	97.3 *	83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	ammonium sulfate, tris-hcl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)	pH7.5
2	1	reservoir	ammonium sulfate	1.6 (M)
3	1	reservoir	Tris	0.1 (M)	pH8.0