1MH1
SMALL G-PROTEIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 101 |
| Collection date | 1994-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 46.440, 103.050, 43.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.380 |
| R-factor | 0.175 * |
| Rwork | 0.175 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 821p |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.543 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.300 | 1.440 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.039 | 0.130 |
| Total number of observations | 312780 * | |
| Number of reflections | 42134 | |
| <I/σ(I)> | 9.8 | 5.6 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.6 * | 4 * | pH 6.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-4 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 18-20 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 25 (mM) | |
| 7 | 1 | reservoir | 10 (mM) | ||
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) |
