1MGW
Crystal structure of RNase Sa3, cytotoxic microbial ribonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.720, 64.720, 69.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.600 * - 2.000 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.21400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgg |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.831 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.306 |
| Number of reflections | 11799 | |
| <I/σ(I)> | 31.6 | 5.8 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 9.5 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 * | 293 | Tris, HCl, HEPES, lithium sulphate, pH 7.6, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 0.10 (M) | pH7.6 |
| 2 | 1 | reservoir | 1.6 (M) | ||
| 3 | 1 | drop | Tris-HCl | 0.05 (M) | pH8.2 |
| 4 | 1 | drop | protein | 7.5 (mg/ml) | |
| 5 | 1 | drop | HEPES | 0.05 (M) | |
| 6 | 1 | drop | 0.8 (M) |
