1MFT
Crystal Structure Of Four-Helix Bundle Model
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.605 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.233, 54.810, 37.168 |
| Unit cell angles | 90.00, 105.08, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.500 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ec5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 17.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.217 |
| Total number of observations | 7203 * | |
| Number of reflections | 3229 | |
| <I/σ(I)> | 6.5 | 3 |
| Completeness [%] | 93.8 | 97.8 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 * | 291 | PEG 300, zinc chloride, bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | reservoir | PEG300 | 26 (%(w/v)) | |
| 3 | 1 | reservoir | twofold excess | ||
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH6.0 |
