1MFR
CRYSTAL STRUCTURE OF M FERRITIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 1998-02 |
| Detector | BRANDEIS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 210.800, 210.800, 328.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1RCD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 20.444 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.920 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.064 | |
| Total number of observations | 249976 * | |
| Number of reflections | 141403 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 70.0 | 27 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | 18 * | pH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Na/K tartrate | 1.0 (M) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | acetate | 0.1 (M) | |
| 5 | 1 | reservoir | Na/K tartrate | 1.0 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
| 7 | 1 | reservoir | 20 (mM) | ||
| 8 | 1 | reservoir | acetate | 0.1 (M) |
