1MFQ
Crystal Structure Analysis of a Ternary S-Domain Complex of Human Signal Recognition Particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 131.180, 131.180, 204.051 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.630 - 3.100 |
| Rwork | 0.229 |
| R-free | 0.26700 |
| Structure solution method | SIRAS |
| Starting model (for MR) | PDB entries 1L9A (RNA) 1JID (SRP19) 1QB2 (SRP54 M-domain) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.070 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.117 * | 0.602 * |
| Number of reflections | 19354 | |
| <I/σ(I)> | 24.3 | 6.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 16.6 | 15.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 300.5 | 14% (w/v) PEG8000, 100mM Na-cacodylate, 400mM lithium sulfate, 80mM magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300.5K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG8000 | ||
| 2 | 1 | 1 | Na-cacodylate | ||
| 3 | 1 | 1 | LiSO4 | ||
| 4 | 1 | 1 | MgCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium acetate | 300 (mM) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 5 | 1 | drop | protein | 5 (mg/ml) | |
| 6 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
| 7 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.5 |
| 8 | 1 | reservoir | 400 (mM) | ||
| 9 | 1 | reservoir | 80 (mM) |
