1MFE

RECOGNITION OF A CELL-SURFACE OLIGO-SACCHARIDE OF PATHOGENIC SALMONELLA BY AN ANTIBODY FAB FRAGMENT

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	47.300, 128.600, 79.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.050 *
R-factor	0.185
Rwork	0.185
RMSD bond length	0.014
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.050 *
Completeness [%]	98.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		taken from Rose, D.R. et al. (1990). J. Mol. Biol., 215, 489-492. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	15 (%(w/v))
3	1	reservoir	Tris-HCl	50 (mM)