1MEY
CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.070, 165.530, 46.274 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.31900 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.860 * |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.088 |
| Total number of observations | 146523 * |
| Number of reflections | 23392 |
| Completeness [%] | 84.0 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 20 * | pH 7.80, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 1500 | ||
| 3 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | bis-tris propane | 25 (mM) | |
| 2 | 1 | reservoir | 10 (mM) | ||
| 3 | 1 | reservoir | 80-100 (mM) | ||
| 4 | 1 | reservoir | PEG1500 | 22-24 (%) |
