1MEX
Antibody Catalysis of a Bimolecular Cycloaddition Reaction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 84 |
| Detector technology | CCD |
| Collection date | 2001-05-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96482 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.320, 83.310, 107.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.250 |
| Rwork | 0.153 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 112412 | |
| <I/σ(I)> | 27.4 | 1.9 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295.5 | MPEG2000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |
