1MEL

CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME

Experimental procedure

Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	298
Detector technology	DIFFRACTOMETER
Collection date	1996-05-02
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	68.680, 69.710, 113.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.221
Rwork	0.221
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1LYS AND 1IGM
RMSD bond length	0.013
RMSD bond angle	24.049 *
Data reduction software	MADNES
Data scaling software	SCALA ((CCP4))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.114	0.420
Number of reflections	17829
<I/σ(I)>	6.1	1.8
Completeness [%]	93.4	84.8
Redundancy	5.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4		HANGING DROP, pH 6.4, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein complex	5 (mg/ml)
2	1	drop	PEG8000	15-20 (%(w/v))
3	1	drop	potassium phosphate	0.1 (M)