1MEJ

Human Glycinamide Ribonucleotide Transformylase domain at pH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-06-20
Detector	MARRESEARCH
Wavelength(s)	0.9706
Spacegroup name	H 3 2
Unit cell lengths	152.510, 152.510, 193.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 2.000
Rwork	0.216
R-free	0.25200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c2t
RMSD bond length	0.009
RMSD bond angle	1.340 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.060	0.372
Total number of observations	118898 *
Number of reflections	53638
<I/σ(I)>		1.6
Completeness [%]	97.8 *	96.8 *
Redundancy	2.21 *	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	4 *	PEG4000, PEG400, NaCl, Tris pH 8.5,, VAPOR DIFFUSION, SITTING DROP, temperature 282K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	18-21 (%)
3	1	reservoir	PEG400	2 (%)
4	1	reservoir		0.1 (M)
5	1	reservoir	Tris	0.1 (M)	pH8.5