1MEG

CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Collection date	1994-11-10
Detector	FUJI
Spacegroup name	C 1 2 1
Unit cell lengths	53.450, 65.330, 64.370
Unit cell angles	90.00, 111.60, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.193
Rwork	0.193
RMSD bond length	0.012
RMSD bond angle	1.700 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	7.720 *
High resolution limit [Å]	2.000 *
Rmerge	0.060
Total number of observations	42446 *
Number of reflections	12010
Completeness [%]	86.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	drop	Tris	10 (mM)
3	1	reservoir	PEG1000	29 (%)
4	1	reservoir		0.3 (M)