1MCO

THREE-DIMENSIONAL STRUCTURE OF A HUMAN IMMUNOGLOBULIN WITH A HINGE DELETION

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	88.400, 110.000, 186.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 3.200
R-factor	0.232
Rwork	0.232
RMSD bond length	0.022
RMSD bond angle	0.027 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	3.200 *
Rmerge	0.148 *
Total number of observations	43520 *
Number of reflections	14525 *
Completeness [%]	94.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8 *	20 *	Edmundson, A.B., (1970) J.Biol.Chem., 245, 2763. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	0.1 (M)
2	1	1	protein	30-60 (mg/ml)
3	1	2	deionized water