1MC3

CRYSTAL STRUCTURE OF RFFH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2000-10-23
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	144.338, 71.682, 59.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
Rwork	0.223
R-free	0.28400 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010 *
RMSD bond angle	1.800 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.088 *
Total number of observations	47293 *
Number of reflections	16531
<I/σ(I)>	9.5
Completeness [%]	90.3	46.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	291	PEG8K 16%, 0.1M MES, 0.4M MGCL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	20 (mM)	pH7.5
3	1	drop		0.2 (M)
4	1	drop	dithiothreitol	5 (mM)
5	1	drop	dTTP	5 (mM)
6	1	reservoir	PEG8000	16 (%(w/v))
7	1	reservoir	MES	0.1 (M)	pH6.5
8	1	reservoir		0.4 (M)