1MBU

Crystal Structure Analysis of ClpSN heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-07
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00930
Spacegroup name	P 31 2 1
Unit cell lengths	87.913, 87.913, 209.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.300
Rwork	0.217
R-free	0.23600 *
Structure solution method	MIR
RMSD bond length	0.012 *
RMSD bond angle	1.460 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.055 *	0.579 *
Number of reflections	42650
<I/σ(I)>	32.4	2.9
Completeness [%]	99.9	100
Redundancy	7.31	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	bis-Tris	0.1 (M)	pH6.5
2	1	reservoir	glycerol	32 (%)
3	1	reservoir	yttrium chloride	10-15 (mM)
4	1	drop	protein	10 (mg/ml)