1MAZ

X-RAY STRUCTURE OF BCL-XL, AN INHIBITOR OF PROGRAMMED CELL DEATH

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-11-01
Detector	RIGAKU
Spacegroup name	P 41 21 2
Unit cell lengths	63.500, 63.500, 110.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.192
Rwork	0.192
R-free	0.25900
RMSD bond length	0.014 *
RMSD bond angle	1.920 *
Data reduction software	HKL
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.055
Number of reflections	11139
Completeness [%]	94.0
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	Tris	50 (mM)
3	1	reservoir	PEG4000	28-31 (%(w/v))
4	1	reservoir	ammonium acetate	0.2 (M)
5	1	reservoir	sodium citrate	0.1 (M)