1MAM

CRYSTAL STRUCTURE TO 2.45 A RESOLUTION OF A MONOCLONAL FAB SPECIFIC FOR THE BRUCELLA A CELL WALL POLYSACCHARIDE ANTIGEN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	75.730, 126.820, 46.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.450
R-factor	0.215
RMSD bond length	0.013
RMSD bond angle	0.034 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.420 *
Rmerge	0.052 *
Total number of observations	37288 *
Number of reflections	15355 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Fab		0.004 ml
2	1	drop	ammonium sulfate	0.12 (M)	0.001 ml
3	1	drop	Tris-HCl	0.5 (M)	0.0008 ml
4	1	drop	PEG8000	20 (%(w/v))	0.0042 ml
5	1	reservoir	PEG8000	34 (%)	0.001 ml
6	1	reservoir	sodium azide		trace amount