1MAL

STRUCTURAL BASIS FOR SUGAR TRANSLOCATION THROUGH MALTOPORIN CHANNELS AT 3.1 ANGSTROMS RESOLUTION

Experimental procedure

Collection date	1994-01-07
Spacegroup name	C 2 2 21
Unit cell lengths	131.890, 214.790, 220.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.100
R-factor	0.217
Rwork	0.217
R-free	0.24000
RMSD bond length	0.020
RMSD bond angle	28.800 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	3.100
Rmerge	0.088
Number of reflections	51039
Completeness [%]	95.5
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	LamB	5 (mg/ml)
2	1	drop	PEG4000	3.6 (%(w/v))
3	1	drop	C8-POE	0.25 (%(w/v))
4	1	drop	beta-OG	0.25 (%(w/v))
5	1	reservoir	PEG4000	25.2 (%(w/v))
6	1	reservoir		0.7 (M)
7	1	reservoir	sodium phosphate	0.14 (M)
8	1	reservoir		3 (mM)