1M9Z

CRYSTAL STRUCTURE OF HUMAN TGF-BETA TYPE II RECEPTOR LIGAND BINDING DOMAIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-07-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0092, 1.0011, 1.0076
Spacegroup name	P 21 21 21
Unit cell lengths	35.471, 40.658, 76.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.000 * - 1.050
R-factor	0.15647
Rwork	0.156
R-free	0.16600 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.480 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	1.090
High resolution limit [Å]	1.050	1.050
Rmerge	0.035 *	0.488 *
Number of reflections	45991 *	2277 *
<I/σ(I)>	56.5
Completeness [%]	87.9	44.3
Redundancy	8.5	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	Boesen, C.C., (2002) Acta Crystallogr., D58, 1214. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG2000	30 (%)
3	1	reservoir	sodium citrate	0.1 (M)	pH5.0