1M9I

Crystal Structure Of Phosphorylation-Mimicking Mutant T356D Of Annexin VI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.971
Spacegroup name	P 43
Unit cell lengths	67.970, 67.970, 204.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.210 - 2.650
R-factor	0.243
Rwork	0.225
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	human annexin VI
RMSD bond length	0.007
RMSD bond angle	1.239 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.089	0.266
Number of reflections	32036
<I/σ(I)>	22.8
Completeness [%]	97.2	99.7
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4	298	0.5M ammonium sulfate, 0.1M imidazole, pH 7.4, Vapor diffusion, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	0.5 (M)
2	1	reservoir	imidazole	0.1 (M)	pH7.4
3	1	drop	protein	15-20 (mg/ml)