1M90
Co-crystal structure of CCA-Phe-caproic acid-biotin and sparsomycin bound to the 50S ribosomal subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-15 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.033 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 211.661, 299.773, 573.684 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.22600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jj2 |
RMSD bond length | 0.006 |
RMSD bond angle | 15.600 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.136 | 0.900 |
Number of reflections | 416857 | |
<I/σ(I)> | 15 | 2 |
Completeness [%] | 94.3 | 100 |
Redundancy | 7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | PEG 6000, NaCl, KCl, NH4Cl, MgCl2, ethylene glycol, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 100K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | KCl | ||
4 | 1 | 1 | NH4Cl | ||
5 | 1 | 1 | MgCl2 | ||
6 | 1 | 1 | ethylene glycol |