1M7X
The X-ray Crystallographic Structure of Branching Enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 116 |
Detector technology | CCD |
Collection date | 2000-03-23 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.475, 102.619, 185.060 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.300 |
Rwork | 0.200 |
R-free | 0.26500 * |
Structure solution method | SAD, SIR |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.450 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.086 * | 0.303 |
Number of reflections | 151550 | |
<I/σ(I)> | 2.6 | |
Completeness [%] | 99.6 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | Hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | Hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | Hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium HEPES | 25 (mM) | pH7.5 |
2 | 1 | drop | protein | 5 (mg/ml) | |
3 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.20 |