1M7N

Crystal Structure of Unactivated APO Insulin-like Growth Factor-1 Receptor Kinase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	200
Detector technology	CCD
Spacegroup name	P 1 21 1
Unit cell lengths	52.880, 78.060, 78.970
Unit cell angles	90.00, 99.17, 90.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.229
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.305 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.700
Rmerge	0.089 *	0.244 *
Total number of observations	59944 *
Number of reflections	17255
Completeness [%]	98.4 *	97.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	4 *	0.1M Tris-HCl, 0.2M MgCl2, 29% PEG 8000, 2% Ethylene glycol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%(w/v))
2	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
3	1	reservoir		200 (mM)