1M7H
Crystal Structure of APS kinase from Penicillium Chrysogenum: Structure with APS soaked out of one dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.601, 83.688, 138.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.000 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ternary structure of APS kinase |
| RMSD bond length | 0.009 |
| RMSD bond angle | 21.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.329 |
| Total number of observations | 621412 * | |
| Number of reflections | 64678 * | |
| <I/σ(I)> | 14.1 | 3.7 |
| Completeness [%] | 97.3 | 91.5 |
| Redundancy | 9.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | Na monobasic phosphate, K dibasic phosphate, succinate, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | 1.7 (M) | ||
| 4 | 1 | reservoir | 0.3 (M) | ||
| 5 | 1 | reservoir | sodium succinate | 0.1 (M) | pH4.0 |
