1M6Z
Crystal structure of reduced recombinant cytochrome c4 from Pseudomonas stutzeri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.03260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.110, 57.560, 83.750 |
| Unit cell angles | 90.00, 104.32, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.350 |
| R-factor | 0.1427 |
| Rwork | 0.140 |
| R-free | 0.19070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1etp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 142653 | |
| Completeness [%] | 93.5 | 87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 298 | 0.2M ammonium acetate, 0.1M sodium citrate pH 5.6, 30%(w/v) PEG 4000, 5%(v/v) glycerol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
