1M6X
Flpe-Holliday Junction Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.779, 116.511, 142.425 |
| Unit cell angles | 90.00, 97.26, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 2.800 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.28600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.324 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 48174 | |
| Completeness [%] | 18.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | PEG 5Kmme, sodium cloride, calcium cloride, HEPES, xylitol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | PEG5000 MME | 13 (%(w/v)) | |
| 6 | 1 | reservoir | HEPES | 20 (mM) | pH7.0 |
| 7 | 1 | reservoir | 100 (mM) | ||
| 8 | 1 | reservoir | 10 (mM) | ||
| 9 | 1 | reservoir | xylitol | 20 (%(w/v)) |
