1M6P
EXTRACYTOPLASMIC DOMAIN OF BOVINE CATION-DEPENDENT MANNOSE 6-PHOSPHATE RECEPTOR
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 92.641, 92.641, 85.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.27600 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 * | 0.340 |
| Total number of observations | 144591 * | |
| Number of reflections | 31187 * | |
| <I/σ(I)> | 10.7 | 2 |
| Completeness [%] | 89.0 | 72.5 |
| Redundancy | 4.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.6 * | 19 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.8 (mg/ml) | |
| 2 | 1 | reservoir | ammonium acetate | 0.20 (M) | |
| 3 | 1 | reservoir | PEG4000 | 25 (%) | |
| 4 | 1 | reservoir | cacodylate | 0.10 (M) | pH6.6 |
