1M6G
Structural Characterisation of the Holliday Junction TCGGTACCGA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.811 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.302, 25.034, 36.778 |
| Unit cell angles | 90.00, 110.04, 90.00 |
Refinement procedure
| Resolution | 34.503 - 1.652 |
| R-factor | 0.2148 |
| Rwork | 0.215 |
| R-free | 0.29566 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.868 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.503 | 1.695 |
| High resolution limit [Å] | 1.649 | 1.652 |
| Rmerge | 0.296 | 0.332 |
| Number of reflections | 6703 | |
| Redundancy | 5.41 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | cacodylate, strontium chloride, MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate | ||
| 2 | 1 | 1 | SrCl2 | ||
| 3 | 1 | 1 | MPD |
