1M5Q
Crystal structure of a novel Sm-like archaeal protein from Pyrobaculum aerophilum
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2002-04-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97954, 0.97933, 0.97186 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.322, 172.427, 148.108 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.000 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.23600 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.740 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.110 | 0.398 |
Total number of observations | 1693038 * | |
Number of reflections | 270937 | |
<I/σ(I)> | 14.5 | 2.9 |
Completeness [%] | 96.3 | 76.3 |
Redundancy | 6.3 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 3350, glycerol, ammonium acetate, sodium acetate, cadmium chloride, sodium chloride, pH 8.0 VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 85 (mg/ml) | |
2 | 1 | reservoir | UMP | 20 (mM) | |
3 | 1 | reservoir | 10 (mM) |