1M5Q

Crystal structure of a novel Sm-like archaeal protein from Pyrobaculum aerophilum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	110
Detector technology	CCD
Collection date	2002-04-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97954, 0.97933, 0.97186
Spacegroup name	P 1 21 1
Unit cell lengths	83.322, 172.427, 148.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.191
Rwork	0.191
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.740 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.110	0.398
Total number of observations	1693038 *
Number of reflections	270937
<I/σ(I)>	14.5	2.9
Completeness [%]	96.3	76.3
Redundancy	6.3	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG 3350, glycerol, ammonium acetate, sodium acetate, cadmium chloride, sodium chloride, pH 8.0 VAPOR DIFFUSION, HANGING DROP at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	85 (mg/ml)
2	1	reservoir	UMP	20 (mM)
3	1	reservoir		10 (mM)