1M5I

Crystal Structure of the coiled coil region 129-250 of the tumor suppressor gene product APC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-04
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 32 2 1
Unit cell lengths	46.047, 46.047, 113.299
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.262
Rwork	0.257
R-free	0.30900
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.067 *	0.127
Total number of observations	65629 *
Number of reflections	9685 *
<I/σ(I)>	18.8	7
Completeness [%]	98.6 *	98.8
Redundancy	6.3	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		4 *	16% PEG 3350, 100mM potassium iodide, 10mM strontium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	14-20 (%)
2	1	reservoir	potassium iodide	100 (mM)
3	1	reservoir		10 (mM)
4	1	drop	protein	20-30 (mg/ml)