1M5I
Crystal Structure of the coiled coil region 129-250 of the tumor suppressor gene product APC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 46.047, 46.047, 113.299 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.262 |
| Rwork | 0.257 |
| R-free | 0.30900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 * | 0.127 |
| Total number of observations | 65629 * | |
| Number of reflections | 9685 * | |
| <I/σ(I)> | 18.8 | 7 |
| Completeness [%] | 98.6 * | 98.8 |
| Redundancy | 6.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 * | 16% PEG 3350, 100mM potassium iodide, 10mM strontium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 14-20 (%) | |
| 2 | 1 | reservoir | potassium iodide | 100 (mM) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | drop | protein | 20-30 (mg/ml) |
