1M4K
Crystal structure of the human natural killer cell activator receptor KIR2DS2 (CD158j)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.535, 97.535, 54.375 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 * - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.221 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | KIR2dL2 KIR2DL1 KIR2DL3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.480 * | |
| Number of reflections | 17624 | |
| <I/σ(I)> | 4.6 | 2.6 |
| Completeness [%] | 61.0 * | 99.6 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | Ammonium sulfate, sodium acetate, Zinc chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH8. |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.3-1.6 (M) | |
| 5 | 1 | reservoir | sodium acetate | 50 (mM) | pH4.6 |
| 6 | 1 | reservoir | 20 (mM) |
