1M45
CRYSTAL STRUCTURE OF MLC1P BOUND TO IQ2 OF MYO2P, A CLASS V MYOSIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.594, 56.455, 56.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.650 |
| R-factor | 0.19284 |
| Rwork | 0.191 |
| R-free | 0.22788 |
| Structure solution method | MOLECULAR REPLACEMENT, Structure of MLC1P bound to IQ 2 AND 3 (SOLVED BY MAD METHOD) |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.365 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.400 | 1.710 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.036 | 0.200 |
| Number of reflections | 17258 | |
| <I/σ(I)> | 25.6 | 6.1 |
| Completeness [%] | 97.1 | 86 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
